NUCLEODUR C18 Gravity液相色谱柱

产品简介
NUCLEODUR C18 Gravity液相色谱柱

C18Gravity：PH稳定应用范围宽达1.0—11.0 
※     满足您对方法开发
※     较端PH应用的需求
※     和复杂样品的分离

      Gravity是在NUCLEODUR**纯硅胶基础上，采用 18%高碳载量和多步交联键合技术。特别适合强碱性样品的分析，而且能在PH=1.0—11.0范围内稳定使用,符合美国药典USP L1，常卖货号760101.46C18 Gravity 4.6X250mm 5um


性能：
主要特点：
NUCLEODUR C18 Gravity液相色谱柱

优越的基失活
方法开发的理想
技术特点：
十八烷基改性高密度、多封端
孔径110A；粒径1.8μm，3 m和5 mμ
10，7，12和16μm粒子的制备要
碳含量18%
推荐应用：
整体复杂的分析分离
复合类分离包括药物，如镇痛药、抗炎药、抗抑郁药；除草剂；植物药物；*抑制剂


Standard program* of columns packed with NUCLEODUR? C18 Gravity
Column system	粒径:	1.8 μm	3 μm	5 μm	10 μm
分析柱	EC columns (standard system, 2–4.6 mm)
 
 +
+	 +
+
制备	VarioPrep columns (VP, ≥ 8 mm)
 
		 +
+

高效液相色谱分析法

订货信息：
							
Length ->	30 mm	50 mm	75 mm	100 mm	125 mm	150 mm	250 mm
NUCLEODUR? C18 Gravity, 1.8 μm
2 mm ID	760078.20	760079.20	760071.20	760076.20		760075.20	
3 mm ID	760078.30	760079.30		760076.30			
4 mm ID	760078.40	760079.40		760076.40			
4.6 mm ID	760078.46	760079.46		760076.46			
EC guard columns*	4 x 2 mm	761901.20		4 x 3 mm	761901.30	
NUCLEODUR? C18 Gravity, 3 μm
2 mm ID		760080.20		760084.20	760081.20	760083.20	760082.20
3 mm ID		760080.30		760084.30	760081.30	760083.30	760082.30
4 mm ID		760080.40		760084.40	760081.40	760083.40	760082.40
4.6 mm ID		760080.46	760086.46	760084.46	760081.46	760083.46	760082.46
EC guard columns*	4 x 2 mm	761902.20		4 x 3 mm	761902.30	
NUCLEODUR? C18 Gravity, 5 μm
2 mm ID		760102.20		760104.20	760100.20	760103.20	760101.20
3 mm ID		760102.30		760104.30	760100.30	760103.30	760101.30
4 mm ID		760102.40		760104.40	760100.40	760103.40	760101.40
4.6 mm ID		760102.46	760106.46	760104.46	760100.46	760103.46	760101.46
EC guard columns*	4 x 2 mm	761903.20		4 x 3 mm	761903.30	


Guard columns for EC columns		EC columns with ID	Use guard 
column holder
			2 mm	3 mm	4 mm	4.6 mm	
*	Column Protection System	EC guard column	4/2	4/3	4/3	4/3	REF 718966

VarioPrep 柱用在高效液相制备


Length	10 mm ID	21 mm ID	32 mm ID	40 mm ID
NUCLEODUR? C18 Gravity, 5 μm
50 mm	762103.100	762103.210		
125 mm	762109.100	762109.210		
150 mm				762100.400
250 mm	762113.100	762113.210	762113.320	762113.400
NUCLEODUR? C18 Gravity, 10 μm
250 mm		762250.210		762250.400
VP guard columns with NUCLEODUR? C18 Gravity
	8 mm ID	16 mm ID	32 mm ID
10 mm*	762160.80	762160.160		
15 mm*			762163.320
* VP guard column	Size	Pack of	for VP column	required holder	REF
VP 10/8	10 x 8 mm ID	2	10 mm ID	8 mm	718251
VP 10/16	10 x 16 mm ID	2	21 mm ID	16 mm	718256
VP 15/32	15 x 32 mm ID	1	32–40 mm ID	32 mm	718253


国家对照品舒巴坦的标定
使用NUCLEODUR C18 Gravity和对照柱X按照《中国药典》方法检测进行标定
色谱柱：NUCLEODUR C18 Gravity 250×4.6mm 5um  对照柱X 250×4.6mm 5um
流动相：0.005 mol/L （PH=5.0）:乙腈=75:25
温  度：35℃
检测器：UV 230nm
结果:
 	舒巴坦理论塔板数	舒巴坦
与杂质分离度	重现性
C18 Gravity	7398	4.13	0.71
对照柱X	6317	4.00	0.15
 



应用：
1、	万古霉素
Substances:		vancomycin
Column:		EC 250/4.6 NUCLEODUR? C18 Gravity, 5 μm
Phase:		NUCLEODUR? C18 Gravity

REF number:		760101.46
		
Sample pretreatment:		Sample concentration: 2 mg/mL in eluent A
Stock solution: 4 mL triethylamine + 1996 mL water + H3PO4 (adjusted to pH 3.2)
		
Conditions:		Eluent A: 920 mL stock solution + 10 mL THF + 70 mL ACN
Eluent B: 700 mL stock solution + 10 mL THF + 290 mL ACN
Gradient: 0 % B for 13 min, 0-100 % B in 9.1 min, 100 % B for 4 min
Flow rate: 1.0 mL/min
Temperature: 25 °C
Injection volume: 20 μL
		
Detection:		UV, 280 nm
		
Author(-s):		HPLC department
Source:		MACHEREY-NAGEL, Germany 2010
		
Keywords:		antibiotics; pharmacopeia; Pharm. Eu.; asymmetry factor
		


Legend:		1  -  vancomycin
Note:		Vancomycin is determined with an asymmetry factor of 1.12 (Pharm. Eu< 1.60)




Substances:		acetylsalicylic acid; sulindac; piroxicam; suprofen; tolmetin; naproxen; diflunisal; fenoprofen; flurbiprofen; niflumic acid; indomethacin; ibuprofen
Column:		EC 250/4.6 NUCLEODUR? C18 Gravity, 5 μm
Phase:		NUCLEODUR? C18 Gravity
MN catalog number:		760101.46
		
		
		
Conditions:		Mobile phase: acetonitrile / 20 mM KH2PO4, pH 2.5, 45:55 (v/v)
Flowrate: 1.3 ml/min
Temperature: 22 °C
Injection volume: 5.0 μl
		
Detection:		UV, 230 nm
		
Author(-s):		HPLC department
Source:		MACHEREY-NAGEL, Germany 2008
		
Keywords:		antiinflamatoray drugs; drugs; NSAID
		





Legend:		1   -  acetylsalicylic acid
2   -  sulindac
3   -  piroxicam
4   -  suprofen
5   -  tolmetin
6   -  naproxen
7   -  diflunisal
8   -  fenoprofen
9   -  flurbiprofen
10 -  niflumic acid
11 -  indomethacin
12 -  ibuprofen



Substances:		coumafuryl; warfarin; pindone; coumatetralyl; coumachlor; diphacinone; chlorphacinone; bromadiolone i; difenacoum; bromadiolone ii; flocoumafen i; flocumafen ii; brodifacoum; difethialone; pyranocoumarin
Column:		EC 250/4.6 NUCLEODUR? C18 Gravity, 5 μm
CC 8/4 NUCLEODUR? C18 Gravity, 5 μm (guard column)
Phase:		NUCLEODUR? C18 Gravity
REF number:		760101.46
761125.40
		
		
		
Conditions:		Buffer:
0.68 g KH2PO4 + 0.34 g Na3PO4 x 12 H20 in 1l dest. water, pH 6.3
Eluents: 
A: Methanol / Acetonitrile / Buffer (250:100:650)
B: Methanol / Acetonitrile / Buffer (100:650:250)
Flow rate: 1.2 ml/min
Temperature: RT
		
Detection:		A) DAD UV, 308 nm (285 nm, 260 nm)
B) Fluorescence (stimulation 308 nm / emission 390 nm)
		
Author(-s):		Freidhof, K.
Source:		LandesuntersuchungsamtRheinland-Pfalz, Koblenz; Germany
		
Keywords:		anti-coagulant drugs; drugs
		

Legend:		Peaks:

A) 
1) Coumafuryl
2) Warfarin &Pindone
3) Coumatetralyl
4) Coumachlor
5) Diphacinone
6) Chlorphacinone
7) Bromadiolone I
8) Difenacoum
9) Bromadiolone II
10) Flocoumafen I
11) Flocumafen II &Brodifacoum
12) Difethialone
13) Pyranocoumarin

B)
1) Coumafuryl
2) Warfarin
3) Coumatetralyl
4) Coumachlor
5) Bromadiolone I
6) Difenacoum
7) Bromadiolone II
8) Flocoumafen&Brodifacoum
9) Pyranocoumarin